A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain.
Identifieur interne : 000283 ( Main/Exploration ); précédent : 000282; suivant : 000284A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain.
Auteurs : Wei-Jie He [République populaire de Chine] ; Meng-Meng Shi [République populaire de Chine] ; Peng Yang [République populaire de Chine] ; Tao Huang [République populaire de Chine] ; Yue Zhao [République populaire de Chine] ; Ai-Bo Wu [République populaire de Chine] ; Wu-Bei Dong [République populaire de Chine] ; He-Ping Li [République populaire de Chine] ; Jing-Bo Zhang [République populaire de Chine] ; Yu-Cai Liao [République populaire de Chine]Source :
- Food chemistry [ 1873-7072 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Hyphomicrobiaceae, Triticum.
- métabolisme : Aldo-keto reductases, Fusarium, NADP, Quinone reductases, Trichothécènes.
- Oxydoréduction, Simulation de docking moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Aldo-Keto Reductases, NADP, Quinone Reductases, Trichothecenes.
- enzymology : Hyphomicrobiaceae, Triticum.
- metabolism : Fusarium.
- Molecular Docking Simulation, Oxidation-Reduction.
Abstract
The Fusarium mycotoxin deoxynivalenol (DON) is typically controlled by fungicides. Here, we report DON detoxification using enzymes from the highly active Devosia strain D6-9 which degraded DON at 2.5 μg/min/108 cells. Strain D6-9 catabolized DON to 3-keto-DON and 3-epi-DON, completely removing DON in wheat. Genome analysis of three Devosia strains (D6-9, D17, and D13584), with strain D6-9 transcriptomes, identified three genes responsible for DON epimerization. One gene encodes a quinone-dependent DON dehydrogenase QDDH which oxidized DON into 3-keto-DON. Two genes encode the NADPH-dependent aldo/keto reductases AKR13B2 and AKR6D1 that convert 3-keto-DON into 3-epi-DON. Recombinant proteins expressed in Escherichia coli efficiently degraded DON in wheat grains. Molecular docking and site-directed mutagenesis revealed that residues S497, E499, and E535 function in QDDH's DON-oxidizing activity. These results advance potential microbial and enzymatic elimination of DON in agricultural samples and lend insight into the underlying mechanisms and molecular evolution of DON detoxification.
DOI: 10.1016/j.foodchem.2020.126703
PubMed: 32247890
Affiliations:
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College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wu, Ai Bo" sort="Wu, Ai Bo" uniqKey="Wu A" first="Ai-Bo" last="Wu">Ai-Bo Wu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Wu Bei" sort="Dong, Wu Bei" uniqKey="Dong W" first="Wu-Bei" last="Dong">Wu-Bei Dong</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Li, He Ping" sort="Li, He Ping" uniqKey="Li H" first="He-Ping" last="Li">He-Ping Li</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhang, Jing Bo" sort="Zhang, Jing Bo" uniqKey="Zhang J" first="Jing-Bo" last="Zhang">Jing-Bo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: jingbozhang@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Liao, Yu Cai" sort="Liao, Yu Cai" uniqKey="Liao Y" first="Yu-Cai" last="Liao">Yu-Cai Liao</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. 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Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Shi, Meng Meng" sort="Shi, Meng Meng" uniqKey="Shi M" first="Meng-Meng" last="Shi">Meng-Meng Shi</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Huang, Tao" sort="Huang, Tao" uniqKey="Huang T" first="Tao" last="Huang">Tao Huang</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhao, Yue" sort="Zhao, Yue" uniqKey="Zhao Y" first="Yue" last="Zhao">Yue Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wu, Ai Bo" sort="Wu, Ai Bo" uniqKey="Wu A" first="Ai-Bo" last="Wu">Ai-Bo Wu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Wu Bei" sort="Dong, Wu Bei" uniqKey="Dong W" first="Wu-Bei" last="Dong">Wu-Bei Dong</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Li, He Ping" sort="Li, He Ping" uniqKey="Li H" first="He-Ping" last="Li">He-Ping Li</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhang, Jing Bo" sort="Zhang, Jing Bo" uniqKey="Zhang J" first="Jing-Bo" last="Zhang">Jing-Bo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: jingbozhang@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Liao, Yu Cai" sort="Liao, Yu Cai" uniqKey="Liao Y" first="Yu-Cai" last="Liao">Yu-Cai Liao</name><affiliation wicri:level="1"><nlm:affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: yucailiao@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Food chemistry</title><idno type="eISSN">1873-7072</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aldo-Keto Reductases (metabolism)</term><term>Fusarium (metabolism)</term><term>Hyphomicrobiaceae (enzymology)</term><term>Molecular Docking Simulation (MeSH)</term><term>NADP (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Quinone Reductases (metabolism)</term><term>Trichothecenes (metabolism)</term><term>Triticum (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aldo-keto reductases (métabolisme)</term><term>Fusarium (métabolisme)</term><term>Hyphomicrobiaceae (enzymologie)</term><term>NADP (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Quinone reductases (métabolisme)</term><term>Simulation de docking moléculaire (MeSH)</term><term>Trichothécènes (métabolisme)</term><term>Triticum (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aldo-Keto Reductases</term><term>NADP</term><term>Quinone Reductases</term><term>Trichothecenes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Hyphomicrobiaceae</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Hyphomicrobiaceae</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aldo-keto reductases</term><term>Fusarium</term><term>NADP</term><term>Quinone reductases</term><term>Trichothécènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Docking Simulation</term><term>Oxidation-Reduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Oxydoréduction</term><term>Simulation de docking moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Fusarium mycotoxin deoxynivalenol (DON) is typically controlled by fungicides. Here, we report DON detoxification using enzymes from the highly active Devosia strain D6-9 which degraded DON at 2.5 μg/min/10<sup>8</sup> cells. Strain D6-9 catabolized DON to 3-keto-DON and 3-epi-DON, completely removing DON in wheat. Genome analysis of three Devosia strains (D6-9, D17, and D13584), with strain D6-9 transcriptomes, identified three genes responsible for DON epimerization. One gene encodes a quinone-dependent DON dehydrogenase QDDH which oxidized DON into 3-keto-DON. Two genes encode the NADPH-dependent aldo/keto reductases AKR13B2 and AKR6D1 that convert 3-keto-DON into 3-epi-DON. Recombinant proteins expressed in Escherichia coli efficiently degraded DON in wheat grains. Molecular docking and site-directed mutagenesis revealed that residues S497, E499, and E535 function in QDDH's DON-oxidizing activity. These results advance potential microbial and enzymatic elimination of DON in agricultural samples and lend insight into the underlying mechanisms and molecular evolution of DON detoxification.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32247890</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-7072</ISSN><JournalIssue CitedMedium="Internet"><Volume>321</Volume><PubDate><Year>2020</Year><Month>Aug</Month><Day>15</Day></PubDate></JournalIssue><Title>Food chemistry</Title><ISOAbbreviation>Food Chem</ISOAbbreviation></Journal><ArticleTitle>A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain.</ArticleTitle><Pagination><MedlinePgn>126703</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0308-8146(20)30565-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.foodchem.2020.126703</ELocationID><Abstract><AbstractText>The Fusarium mycotoxin deoxynivalenol (DON) is typically controlled by fungicides. Here, we report DON detoxification using enzymes from the highly active Devosia strain D6-9 which degraded DON at 2.5 μg/min/10<sup>8</sup> cells. Strain D6-9 catabolized DON to 3-keto-DON and 3-epi-DON, completely removing DON in wheat. Genome analysis of three Devosia strains (D6-9, D17, and D13584), with strain D6-9 transcriptomes, identified three genes responsible for DON epimerization. One gene encodes a quinone-dependent DON dehydrogenase QDDH which oxidized DON into 3-keto-DON. Two genes encode the NADPH-dependent aldo/keto reductases AKR13B2 and AKR6D1 that convert 3-keto-DON into 3-epi-DON. Recombinant proteins expressed in Escherichia coli efficiently degraded DON in wheat grains. Molecular docking and site-directed mutagenesis revealed that residues S497, E499, and E535 function in QDDH's DON-oxidizing activity. These results advance potential microbial and enzymatic elimination of DON in agricultural samples and lend insight into the underlying mechanisms and molecular evolution of DON detoxification.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>Wei-Jie</ForeName><Initials>WJ</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Meng-Meng</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Ai-Bo</ForeName><Initials>AB</Initials><AffiliationInfo><Affiliation>Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Wu-Bei</ForeName><Initials>WB</Initials><AffiliationInfo><Affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>He-Ping</ForeName><Initials>HP</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jing-Bo</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: jingbozhang@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Yu-Cai</ForeName><Initials>YC</Initials><AffiliationInfo><Affiliation>Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: yucailiao@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Chem</MedlineTA><NlmUniqueID>7702639</NlmUniqueID><ISSNLinking>0308-8146</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000609395">3-keto-deoxynivalenol</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014255">Trichothecenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>53-59-8</RegistryNumber><NameOfSubstance UI="D009249">NADP</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.-</RegistryNumber><NameOfSubstance UI="D000074408">Aldo-Keto Reductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.99.-</RegistryNumber><NameOfSubstance UI="D011808">Quinone Reductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>JT37HYP23V</RegistryNumber><NameOfSubstance UI="C007262">deoxynivalenol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000074408" MajorTopicYN="N">Aldo-Keto Reductases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D041921" MajorTopicYN="N">Hyphomicrobiaceae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011808" MajorTopicYN="N">Quinone Reductases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014255" MajorTopicYN="N">Trichothecenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">3-epi-deoxynivalenol (PubChem CID 13456592)</Keyword><Keyword MajorTopicYN="N">Alcohol dehydrogenase</Keyword><Keyword MajorTopicYN="N">Aldo/keto reductases</Keyword><Keyword MajorTopicYN="N">Deoxynivalenol (PubChem CID 40024)</Keyword><Keyword MajorTopicYN="N">Deoxynivalenol epimerization</Keyword><Keyword MajorTopicYN="N">Enzymatic detoxification</Keyword><Keyword MajorTopicYN="N">Molecular docking</Keyword><Keyword MajorTopicYN="N">NADPH (PubChem CID 5884)</Keyword><Keyword MajorTopicYN="N">Pyrroloquinoline quinone (PubChem CID 1024)</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32247890</ArticleId><ArticleId IdType="pii">S0308-8146(20)30565-3</ArticleId><ArticleId IdType="doi">10.1016/j.foodchem.2020.126703</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="He, Wei Jie" sort="He, Wei Jie" uniqKey="He W" first="Wei-Jie" last="He">Wei-Jie He</name></region><name sortKey="Dong, Wu Bei" sort="Dong, Wu Bei" uniqKey="Dong W" first="Wu-Bei" last="Dong">Wu-Bei Dong</name><name sortKey="Huang, Tao" sort="Huang, Tao" uniqKey="Huang T" first="Tao" last="Huang">Tao Huang</name><name sortKey="Li, He Ping" sort="Li, He Ping" uniqKey="Li H" first="He-Ping" last="Li">He-Ping Li</name><name sortKey="Liao, Yu Cai" sort="Liao, Yu Cai" uniqKey="Liao Y" first="Yu-Cai" last="Liao">Yu-Cai Liao</name><name sortKey="Shi, Meng Meng" sort="Shi, Meng Meng" uniqKey="Shi M" first="Meng-Meng" last="Shi">Meng-Meng Shi</name><name sortKey="Wu, Ai Bo" sort="Wu, Ai Bo" uniqKey="Wu A" first="Ai-Bo" last="Wu">Ai-Bo Wu</name><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><name sortKey="Zhang, Jing Bo" sort="Zhang, Jing Bo" uniqKey="Zhang J" first="Jing-Bo" last="Zhang">Jing-Bo Zhang</name><name sortKey="Zhao, Yue" sort="Zhao, Yue" 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